Bacterial Conjugation Experiment

BACTERIAL CONJUGATION EXPERIMENT INTRODUCTION: Bacteria, in general, reproduce asexually, but in order to increase diversity, they have developed a mechanism for transfer of genetic material from one bacterium to another. The ability to perform this transfer is conferred by a set of genes which are called F for 'fertility.' These genes exist on a small, circular piece of deoxyribonucleic acid (DNA) that replicates independently from the bacterial chromosome, or they can be integrated into …showed first 75 words of 1195 total…

…showed last 75 words of 1195 total…minutes 14 NG TNTC NG Histadine deficient plates Time 10-1 10-2 10-3 10-4 0 minutes 10 2 NG NG 10 minutes 46 43 26 3 20 minutes TNTC 136 NG 4 30 minutes TNTC 184 8 3 40 minutes TNTC 340 20 NG 50 minutes 45 TNTC 27 3 60 minutes 41 250 42 NG TNTC = Too numerous to count NG = No growth Figure 4 - Time of entry table (percentages of recombinants) Time Thr - Pro - His - 0 minutes .44 0 0 10 minutes 5.8 2 .02 20 minutes 6.7 2.6 .06 30 minutes .004 1.8 .08 40 minutes .0098 4.3 .09 50 minutes 1.9 2.3 .002 60 minutes 2.4 0 .11 Figure 5 - Time of entry graph (percentages of recombinants) Figure 6 - Map of E. coli K12